Multi-species (Ovine, Caprine) M-CSF Recombinant Protein

Catalog Number:: RP1640G
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Capra hircus (goat), Ovis aries (sheep)

Product Description

Ovine/Caprine M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) (catalog RP1640G) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.0 kDa, 154 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat and sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study M-CSF signaling and hematopoiesis (including regulation of macrophage differentiation, proliferation, and survival); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	EEVSENCSHM IGNGHLLFLQ QLIDSQMETS CQISFEFVDQ EQLDDPVCYL KKAFLLVQDI MEDTMRFKDN TPNAKAIVQL QELSLRLKSC FTMDYDEQDK ACVRTFHETP LQLLEKIKNV FNETKNLLKK DWNVFSKNCN NSFAKCSSHG HERQ (154)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	18.0 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Ovine/Caprine Macrophage Colony-Stimulating Factor (M-CSF) is a cytokine that regulates the development, proliferation, and survival of monocytes and macrophages in sheep (Ovis aries) and goats (Capra hircus). The M-CSF protein is identical at the amino acid level in these two species, reflecting strong evolutionary conservation of this key regulator of macrophage biology in small ruminants. M-CSF is produced by several cell types, including fibroblasts, endothelial cells, epithelial cells, and stromal cells, in response to tissue signals and inflammatory stimuli. Ovine and caprine M-CSF bind to the colony-stimulating factor-1 receptor (CSF1R), a receptor tyrosine kinase expressed on monocytes and macrophage lineage cells, activating intracellular signaling pathways such as PI3K-AKT, ERK/MAPK, and JAK-STAT that promote differentiation and maintenance of macrophage populations. Through these mechanisms, M-CSF supports macrophage-mediated immune surveillance, phagocytosis, tissue homeostasis, and inflammatory responses. Because macrophages play a central role in host defense, M-CSF-mediated macrophage function is important for maintaining immune health and resistance to infection in sheep and goats. In cell culture, recombinant ovine/caprine M-CSF is commonly used to promote differentiation of monocytes or bone marrow-derived cells into macrophages and to support macrophage proliferation and functional assays in vitro. Ovine and caprine M-CSF are also studied in animal model and veterinary research, including investigations of macrophage responses to pathogens such as Mycobacterium avium subsp. paratuberculosis and small ruminant lentiviruses.

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