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Bovine IL-25 (IL-17E) Recombinant Protein
- Catalog Number:
- RP1646B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bison bison bison (American buffalo), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)
- Bovine IL-25 (IL-17E) (catalog RP1646B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.5 kDa, 153 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, American buffalo, zebu, and wild yak. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-25/IL-17E signaling and immune responses (including regulation of type 2 immunity, inflammation, and cytokine production); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | LCLGSQRCCT HWPSCCPSKG QNSTEEWLKW NSVLMPPPET TSLAHHPEPC SSSRDGPLNS RSIAPWRYEL DRDLNRLPQD LYHARCLCPH CVSLQTGSHM DPLGNSELLY HNQTVFYRRP CPGQQGDHHG YCLERRLYRV SLACVCVRPR VMA (153) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.5 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine interleukin-25 (IL-25), also known as IL-17E, is a member of the IL-17 cytokine family and functions as a regulator of type 2 immune responses through interaction with the IL-17RA/IL-17RB receptor complex, activating downstream NF-κB and MAPK signaling pathways. In cattle, IL-25 is expected to promote Th2-associated cytokine production, including IL-4, IL-5, and IL-13, supporting eosinophil recruitment, mucosal immunity, and anti-helminth defense-processes that are particularly important in response to gastrointestinal nematodes and other parasitic infections common in livestock. In bovine health, IL-25 likely plays a role in balancing protective immunity and inflammation in conditions such as parasitic gastroenteritis, respiratory disease complex, mastitis-associated inflammatory responses, and allergic or hypersensitivity reactions. Dysregulated IL-25 signaling may contribute to excessive type 2 polarization, impacting productivity, growth, and overall herd health. Given the economic and agricultural importance of cattle, bovine IL-25 is relevant for understanding host-parasite interactions, improving vaccine strategies, and developing immunomodulatory approaches to enhance disease resistance, while also serving as a comparative model for studying type 2-driven inflammatory diseases across mammalian species.
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