Transforming Growth Factor Alpha (TGF-α) is a member of the epidermal growth factor (EGF) family of growth factors, which also includes epidermal growth factor (EGF), amphiregulin, betacellulin, epiregulin, and heparin-binding EGF-like growth factor (HB-EGF). Rabbit, human, and monkey TGF-α share 100% amino-acid sequence identity and are synthesized as membrane-bound precursors (pro–TGF-α) that are proteolytically cleaved to release a soluble ligand capable of binding the epidermal growth factor receptor (EGFR/ErbB1). Activation of EGFR initiates intracellular signaling pathways including MAPK/ERK, PI3K/AKT, and JAK/STAT, which regulate cell proliferation, differentiation, epithelial growth, and tissue repair. TGF-α is expressed in multiple tissues, including the skin, gastrointestinal tract, liver, and reproductive tissues, where it contributes to epithelial cell turnover, organ development, and regeneration following tissue injury. TGF-α signaling has been studied in models of wound healing, epithelial regeneration, and cancer biology, where EGFR-mediated signaling influences cell proliferation and tissue remodeling. Because rabbit, human, and monkey TGF-α are 100% identical at the amino-acid level, this growth factor is particularly useful for comparative studies of EGFR signaling, epithelial biology, regenerative processes, and growth factor–mediated tissue repair across veterinary, translational, and human biomedical research.