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Rat CD40 Ligand (TNFSF5) Recombinant Protein
- Catalog Number:
- RP1650R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat CD40 Ligand (CD40L, CD154) (catalog RP1650R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.4 kDa, 149 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CD40L signaling and immune responses (including regulation of B-cell activation, antibody production, and T-cell-mediated immunity); typical experimental uses include cell-culture stimulation and activation assays, immune signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MQKGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VVLENGRQLT VKREGLYYVY TQVTFCSNRE PLSQRPFIVS LWLKPSSGSE RILLRAANTH SSSKLCEQQS IHLGGVFELQ AGASVFVNVT EASQVIHGIG FSSFGLLKL (149) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.4 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat CD40 Ligand (CD40L), also known as CD154 and encoded by the Cd40lg gene, is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and intercellular signaling. In rats (Rattus norvegicus), CD40L is primarily expressed on activated CD4⁺ T lymphocytes, and can also be detected on platelets and other activated immune cells following antigen stimulation. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, triggering intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, proliferation, immunoglobulin class switching, germinal center formation, and the development of memory B cells. Through these mechanisms, rat CD40L plays a key role in T cell-dependent antibody responses and regulation of adaptive immunity. Because rats are widely used as experimental models for immunology, autoimmune disease, transplantation, and inflammatory disorders, rat CD40L has been studied in models of immune activation, chronic inflammation, and immune-mediated diseases, helping to clarify mechanisms of costimulatory signaling and adaptive immune regulation relevant to both basic immunology and translational biomedical research.
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