Human IL-33 Recombinant Protein

Catalog Number:
RP1652H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Homo sapiens (human), Pan troglodytes (chimpanzee)
  • Human IL-33 (Interleukin-33) (catalog RP1652H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.0 kDa, 159 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human and chimpanzee. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-33 signaling and immune responses (including roles in inflammation, type 2 immunity, and cytokine-mediated signaling pathways); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET (159)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight18.0 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human Interleukin-33 (IL-33) is a cytokine that plays an important role in regulating immune responses and inflammatory signaling in humans (Homo sapiens). IL-33 is a member of the IL-1 cytokine family and is produced primarily by epithelial cells, endothelial cells, and fibroblasts, particularly in barrier tissues such as the skin, lungs, and gastrointestinal tract. It functions as an “alarmin,” being released upon cellular damage or stress to alert and activate the immune system. Human IL-33 binds to the ST2 receptor (IL1RL1) together with the IL-1 receptor accessory protein (IL-1RAcP), activating intracellular signaling pathways such as NF-κB and MAPK that promote production of inflammatory mediators. IL-33 plays a key role in promoting type 2 immune responses by activating T helper 2 (Th2) cells, group 2 innate lymphoid cells (ILC2s), mast cells, eosinophils, and basophils, leading to the production of cytokines such as IL-4, IL-5, and IL-13. Through these actions, IL-33 contributes to regulation of allergic inflammation, tissue repair, and host defense against parasites. In humans, IL-33 has been strongly associated with inflammatory and allergic diseases including asthma, atopic dermatitis, allergic rhinitis, and other Th2-mediated disorders, and it is widely studied in immunology and translational biomedical research as a therapeutic target for inflammatory and immune-mediated diseases.

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