Rabbit M-CSF Recombinant Protein

Catalog Number:
RP1656U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) (catalog RP1656U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~26.1 kDa, 230 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study M-CSF signaling and hematopoiesis (including regulation of macrophage differentiation, proliferation, and survival); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceEEISEHCSHM IGNGHLQALQ HLIDSQMETS CHITFEFADE EQLKDPVCYL KKAFLLVDDI MEDTMRFRDE TPNAKAIIQL QTLSVRLKSC FTKDYEKQDK ACVRTFYETP LQLMEKIKNV FNETKNLLEE DWKIFTKNCS DSFAKCSSPD VVTKPDCNCL YPKATPSSDL ASVSPHQPFI PSKVPKTGLT WADSEGTEGR SLLPNKQLLH TVDPGTAKHR PPRSTCQSLE (230)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight17.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Macrophage Colony-Stimulating Factor (M-CSF) is a cytokine that regulates the development, proliferation, and survival of monocytes and macrophages in rabbits (Oryctolagus cuniculus). M-CSF is produced by several cell types, including fibroblasts, endothelial cells, epithelial cells, and stromal cells, in response to tissue signals and inflammatory stimuli. Rabbit M-CSF binds to the colony-stimulating factor-1 receptor (CSF1R), a receptor tyrosine kinase expressed on monocytes and macrophage lineage cells, activating intracellular signaling pathways such as PI3K–AKT, ERK/MAPK, and JAK–STAT that promote differentiation and maintenance of macrophage populations. Through these mechanisms, M-CSF supports macrophage-mediated immune surveillance, phagocytosis, tissue homeostasis, and inflammatory responses. Because macrophages play a central role in host defense and immune regulation, M-CSF–mediated macrophage function is important for maintaining immune health and responding to infection or tissue injury in rabbits. In cell culture, recombinant rabbit M-CSF is commonly used to promote differentiation of rabbit monocytes or bone marrow–derived cells into macrophages and to support macrophage proliferation and functional assays in vitro. Rabbit M-CSF is also used in rabbit animal model research; for example, rabbit models of atherosclerosis are used to study macrophage-driven plaque formation and inflammatory processes involved in cardiovascular disease.

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1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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