Turkey CCL4 (MIP-1 beta) Recombinant Protein

Catalog Number:
RP1662T
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Meleagris gallopavo (turkey)
  • Turkey CCL4 (MIP-1β, Macrophage Inflammatory Protein-1 beta) (catalog RP1662T) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to turkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL4/MIP-1β signaling and immune-cell chemotaxis (including recruitment and activation of T cells, monocytes, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPVGSDPPTS CCFTFVSRQI PFNFVADYYE TNSQCPHAGV VFITKKGREV CANPENDWVQ DYMNKLELN (69)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids69
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Turkey CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta–like chemokine in birds) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in turkeys (Meleagris gallopavo). CCL4 functions through chemokine receptors homologous to mammalian CCR5, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer–like cells, and activated T lymphocytes to sites of infection or tissue injury. In turkeys, CCL4 is produced by activated macrophages, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecules and cytokines including IL-1β and TNF-like mediators. CCL4-mediated immune cell recruitment plays an important role in host defense against pathogens responsible for avian influenza virus, Newcastle disease virus, turkey hemorrhagic enteritis virus, and bacterial infections such as Mycoplasma gallisepticum. By directing leukocyte trafficking to infected tissues, CCL4 helps coordinate innate and adaptive immune responses and contributes to pathogen clearance. However, excessive or prolonged expression may contribute to inflammatory tissue damage. As a regulator of leukocyte migration and inflammatory signaling, turkey CCL4 is important for studies of avian host–pathogen interactions, immune regulation, and vaccine responses, supporting research aimed at improving disease resistance and flock health in commercial turkey production systems.
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