Anole IGF-2 Recombinant Protein

Catalog Number:: RP1676A
Application:: Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:: Anolis sagrei ordinatus (brown anole)

Product Description

Anole IGF-2 (Insulin-like Growth Factor 2) (catalog RP1676A) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.6 kDa, 68 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to brown anole. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IGF-2 signaling and cellular processes (including regulation of cell growth, proliferation, differentiation, and development); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Properties/Specifications

Amino Acid Sequence	SHGPAETLCG GELVDTLQFV CGDRGFYFSR PVGRNRGRLN RGIVEECCFR SCDLNLLETY CAKSVKSE (68)
Endotoxin	Naturally endotoxin-free
Form	Lyophilized
Storage Conditions	-20°C
Molecular Weight	7.6 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Affinity Tag	None
aminoAcids	68
Carrier Protein	None
Expression System	Yeast
Form	Lyophilized
Country Of Origin	USA

Anole Insulin-like Growth Factor 2 (IGF-2) is a peptide growth factor involved in regulating embryonic development, tissue growth, and metabolic processes in anole lizards such as the green anole (Anolis carolinensis). IGF-2 is expressed in multiple tissues during development and in certain adult tissues, where it contributes to cell proliferation, differentiation, and survival. Anole IGF-2 binds primarily to the insulin-like growth factor 1 receptor (IGF1R) and can also interact with the insulin receptor, activating intracellular signaling pathways including PI3K–AKT and MAPK that regulate gene expression, cell growth, and metabolic activity. In anoles, IGF-2 is believed to play an important role during embryogenesis, organ formation, and early postnatal growth, and it may also contribute to tissue maintenance and regeneration in adult reptiles. Due to these functions, IGF-2 is studied in reptile developmental biology and comparative endocrinology as a key regulator of growth signaling pathways and vertebrate developmental processes.