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Swine IL-5 Recombinant Protein
- Catalog Number:
- RP1683S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine IL-5 (Interleukin-5) (catalog RP1683S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.0 kDa, 113 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-5 signaling and immune responses (including regulation of eosinophil growth, differentiation, and activation, as well as roles in allergic inflammation); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | VENTMNRLVA ETLTLLSIHR TLLIGDGNLM ISTPVHTNHQ LCIEEVFQGI DTLKNQTARG DAVEKLFQNL SLIKEYIDRQ KKNCGGERWR VTQFLDYLQV FLGVINTEWT MES (113) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine Interleukin-5 (IL-5) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and type 2 innate lymphoid cells in pigs (Sus scrofa domesticus), where it plays a central role in eosinophil differentiation, activation, recruitment, and survival. IL-5 belongs to the common beta chain (βc) cytokine family, which also includes IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF); these cytokines share the βc receptor subunit and regulate hematopoiesis and myeloid cell function. IL-5 signals through a receptor complex composed of IL-5Rα and the shared βc chain, activating JAK/STAT and MAPK pathways that promote eosinophil maturation in the bone marrow and enhance their effector activity in peripheral tissues. In swine health, IL-5 is particularly important in immune responses to parasitic infections such as Ascaris suum and other helminths, where Th2 polarization and eosinophil activation contribute to parasite clearance and mucosal repair. IL-5 may also influence allergic-type inflammation and respiratory immune responses, affecting airway inflammation and tissue remodeling. While IL-5-mediated responses are protective in helminth defense, excessive or prolonged IL-5 signaling can drive persistent eosinophilic inflammation and tissue damage. As a biomarker of type 2 immune activation, swine IL-5 is relevant in vaccine development, host-parasite interaction studies, and immune resilience research. In translational research, pigs serve as valuable large-animal models due to physiological similarities to humans, and characterization of swine IL-5 supports comparative investigations into eosinophil biology and development of targeted anti-IL-5 therapeutic strategies for allergic and inflammatory diseases.
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