Mouse CXCL4 (PF-4) Recombinant Protein

Catalog Number:
RP1712M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus caroli (Ryukyu mouse), Mus musculus (house mouse)
  • Mouse CXCL4 (Platelet Factor 4, PF4) (catalog RP1712M) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.2 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse and Ryukyu mouse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL4/PF4 signaling and immune responses (including roles in platelet activation, inflammation, and chemotaxis); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES (76)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse CXCL4 (C-X-C motif chemokine ligand 4), also known as Platelet Factor 4 (PF4), is a CXC chemokine belonging to the platelet chemokine subgroup of the CXC chemokine family and is primarily released from the α-granules of activated platelets. In mice (Mus musculus), CXCL4 is produced mainly by platelets and megakaryocytes and is rapidly released during platelet activation, vascular injury, or inflammatory responses. CXCL4 plays important roles in hemostasis, modulation of inflammatory signaling, and regulation of leukocyte recruitment, influencing the activity of monocytes, neutrophils, macrophages, and endothelial cells through interactions with cell-surface glycosaminoglycans and chemokine receptors. In murine biology, CXCL4 has been widely studied in experimental models of thrombosis, atherosclerosis, and inflammatory disease, where platelet-derived CXCL4 contributes to platelet–leukocyte interactions, vascular inflammation, and development of atherosclerotic lesions. Mouse models lacking Cxcl4/Pf4 or using platelet-specific manipulations have helped clarify how CXCL4 regulates immune–coagulation crosstalk and vascular inflammation, making mouse CXCL4 an important molecule in studies of platelet biology, cardiovascular disease, and inflammatory signaling.

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