Cynomolgus Monkey CXCL4 (PF-4) Recombinant Protein

Catalog Number:
RP1714Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Piliocolobus tephrosceles (Ugandan red Colobus), Rhinopithecus bieti (black snub-nosed monkey), Rhinopithecus roxellana (golden snub-nosed monkey), Theropithecus gelada (gelada)
  • Cynomolgus Monkey CXCL4 (Platelet Factor 4, PF4) (catalog RP1714Y) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 71 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, sooty mangabey, green monkey, drill, rhesus monkey, pig-tailed macaque, Ugandan red colobus, golden snub-nosed monkey, black snub-nosed monkey, and gelada. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL4/PF4 signaling and immune responses (including roles in platelet activation, inflammation, and chemotaxis); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIVKKLLE S (71)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) CXCL4 (C-X-C motif chemokine ligand 4), also known as Platelet Factor 4 (PF4), is a CXC chemokine belonging to the platelet chemokine subgroup of the CXC chemokine family and is primarily released from α-granules of activated platelets. In cynomolgus monkeys, CXCL4 is produced mainly by platelets and megakaryocytes and is rapidly released during platelet activation, vascular injury, or inflammatory responses. CXCL4 plays important roles in hemostasis, inflammation, and immune modulation, influencing the behavior of monocytes, neutrophils, and other leukocytes, and interacting with glycosaminoglycans on endothelial cells and platelets to regulate platelet aggregation, vascular repair, and leukocyte recruitment at sites of tissue damage. Because cynomolgus monkeys share close genetic, physiological, and immunological similarities with humans, CXCL4 in this species is commonly studied in preclinical models of thrombosis, cardiovascular disease, and inflammatory disorders, including investigations of platelet activation and immune–coagulation interactions. As a result, cynomolgus monkey CXCL4 is important in translational biomedical research, particularly in studies evaluating antithrombotic therapies, inflammatory signaling pathways, and platelet-mediated immune responses relevant to human disease.

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