Ferret IL-15 Recombinant Protein

Catalog Number:
RP1721FT
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mustela putorius furo (domestic ferret)
  • Ferret IL-15 (Interleukin-15) (catalog RP1721FT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.0 kDa, 133 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic ferret. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-15 signaling and immune responses (including regulation of NK cell and T-cell proliferation, survival, and activation); typical experimental uses include cell-culture stimulation and proliferation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGIHVFILGSI SAGLPKTEAK WEFVIKDLEK IDKIIQSIHI DTTLYTESDA HPGCKVTAMN CFLLELRVVL LEFSHANLLN ETIHNIIIQA NSNLSSNANI TETGCKECEE LEEKNIKEFF QSFLHIVQIF HNS (133)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight15.0 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids133
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret Interleukin-15 (IL-15) is a cytokine that plays an important role in regulating innate and adaptive immune responses in ferrets (Mustela putorius furo). IL-15 is produced by a variety of cell types, including monocytes, macrophages, dendritic cells, and epithelial cells, in response to infection or inflammatory stimuli. Ferret IL-15 signals through a receptor complex composed of IL-15Rα together with the shared IL-2/15Rβ (CD122) and common γ chain (γc), activating intracellular signaling pathways such as JAK–STAT that promote lymphocyte proliferation, survival, and activation. In ferrets, IL-15 is particularly important for the development, maintenance, and activation of natural killer (NK) cells and memory CD8⁺ T cells, supporting cytotoxic immune responses against infected cells. It also contributes to regulation of mucosal immunity in respiratory tissues. In cell culture, recombinant ferret IL-15 is commonly used to stimulate proliferation and survival of NK cells and cytotoxic T lymphocytes, maintain memory CD8⁺ T cell populations, and enhance cytotoxic activity during in vitro immune assays. Ferret IL-15 is widely studied in infectious disease and translational research because ferrets serve as an important animal model for human respiratory viruses; for example, ferret models of influenza and SARS-CoV-2 infection are used to investigate immune responses, including IL-15–mediated activation of cytotoxic lymphocytes, to better understand antiviral immunity and evaluate vaccine and therapeutic strategies relevant to human disease.
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