Multi-species (Ferret, Mink) CCL3 (MIP-1 alpha) Recombinant Protein

Catalog Number:
RP1733FT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mustela erminea (ermine), Mustela putorius furo (domestic ferret), Neogale vison (American mink)
  • Ferret/Mink CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1733FT) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic ferret, American mink, and ermine. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune responses (including recruitment and activation of macrophages, T cells, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPFGADTPTA CCFSYVSKQI TRKFIVDYFE TSSQCSKPGV IFQTRKGRQL CADPTEDWVQ EYVTDLELNA (70)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during innate and adaptive immune responses in ferrets (Mustela putorius furo). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In ferrets, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecules and cytokines including TNF-α and IL-1β. In ferret health and research, CCL3 is particularly relevant in respiratory viral infection models, including influenza virus and SARS-CoV-2, where it contributes to recruitment of inflammatory immune cells to the lungs and helps coordinate antiviral immune responses. While CCL3-mediated leukocyte trafficking supports pathogen clearance and initiation of immune defense, excessive expression can contribute to inflammatory lung pathology. Because ferrets are widely used as translational models for human respiratory infections and immune responses, characterization of ferret CCL3 supports studies of chemokine-mediated immune cell migration, host–pathogen interactions, and inflammatory mechanisms relevant to respiratory disease and antiviral immunity.

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