Duck IL-8 Recombinant Protein

Catalog Number:
RP1742MD
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Anas platyrhynchos (mallard)
  • Mallard IL-8 (CXCL8, Interleukin-8) (catalog RP1742MD) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.2 kDa, 82 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to mallard. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-8/CXCL8 signaling and immune responses (including recruitment and activation of neutrophils during inflammatory responses); typical experimental uses include cell-culture stimulation and chemotaxis assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceRSLVRMGNEL RCQCISTHSK FIHPKSIQDV KLTQSGPHCK NVEIIATLKD NREVCLDPTA PWVQRIVKAI LAKAQLNSDA PL (82)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight9.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mallard (Anas platyrhynchos) Interleukin-8 (IL-8), also referred to as CXCLi2 in avian species, is a key pro-inflammatory chemokine that regulates heterophil chemotaxis and activation in response to infection and tissue injury. Functionally analogous to mammalian IL-8, mallard IL-8 is produced by macrophages, epithelial cells, and other immune cells following stimulation by viral, bacterial, or parasitic pathogens and signals through CXCR1- and CXCR2-like receptors to promote recruitment of heterophils—the avian equivalent of neutrophils—to affected tissues. As natural reservoir hosts for avian influenza viruses (AIV), mallards are particularly important for studying IL-8–mediated inflammatory responses, as they often mount effective antiviral defenses while exhibiting limited clinical disease. IL-8 expression is also induced during infections with Salmonella, Campylobacter, and other waterborne pathogens, contributing to mucosal immune responses in respiratory and gastrointestinal tissues. In animal model research, mallards serve as critical models for avian influenza ecology, viral transmission dynamics, and host tolerance mechanisms, and characterization of IL-8 in this species enhances understanding of chemokine-driven innate immunity, heterophil trafficking, and inflammatory regulation relevant to both wildlife disease surveillance and comparative immunology.

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