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Ovine CCL28 Recombinant Protein
- Catalog Number:
- RP1764V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Ovis aries (sheep)
- Ovine CCL28 (MEC, Mucosae-Associated Epithelial Chemokine) (catalog RP1764V) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.1 kDa, 105 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL28/MEC signaling and immune responses (including regulation of mucosal immunity, IgA-secreting cell trafficking, and lymphocyte homing to epithelial tissues); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | ILPIASSCCT EVSHHVSRRL LERVTTCRIQ RADGDCDLAA VILHVKRRRV CVSPHNHVIK QWMKEQAAKK QAQGNVCHKK RHHARRNSKG PHRERQEAHG HKTPY (105) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 12.1 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine CCL28 (C-C motif chemokine ligand 28, also known as MEC - mucosae-associated epithelial chemokine) is a mucosal chemokine that plays an important role in immune cell trafficking and mucosal immune defense in sheep (Ovis aries). CCL28 primarily signals through the CCR10 receptor and, to a lesser extent, CCR3, promoting chemotaxis of IgA-secreting plasma cells, memory T cells, and certain dendritic cell populations to mucosal tissues. In sheep, CCL28 is predominantly produced by epithelial cells at mucosal surfaces, including the intestinal tract, respiratory epithelium, mammary gland, and salivary glands, where it helps recruit IgA-producing plasma cells and supports secretory IgA-mediated immunity at barrier tissues. CCL28-mediated immune cell trafficking is particularly important for defense against gastrointestinal parasites, bacterial enteric infections, and respiratory pathogens, which commonly affect small ruminants. In addition, CCL28 plays a role in the mammary gland during lactation, helping direct IgA-producing cells to mammary tissue and supporting passive immune protection for newborn lambs through colostrum and milk. Proper regulation of CCL28 supports mucosal immune surveillance and pathogen defense, while dysregulation may contribute to mucosal inflammation. Characterization of ovine CCL28 supports research into mucosal immunity, host-pathogen interactions, and vaccine strategies aimed at improving disease resistance and health in sheep production systems.
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