Ferret CCL8 (MCP-2) Recombinant Protein

Catalog Number:
RP1798FT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mustela putorius furo (domestic ferret)
  • Ferret CCL8 (MCP-2, Monocyte Chemoattractant Protein-2) (catalog RP1798FT) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.6 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic ferret. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL8/MCP-2 signaling and immune responses (including recruitment and activation of monocytes, lymphocytes, and other leukocytes during inflammatory processes); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQPGSVSIPIT CCFNVVKRKV PLQKLESYTR VTNSQCPQEA VIFKTKASKE ICADPQLKWV QDNMKHLDQK SSTQKP (76)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.6 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret CCL8 (C–C motif chemokine ligand 8, also known as MCP-2 – Monocyte Chemoattractant Protein-2) is a proinflammatory chemokine involved in leukocyte recruitment and regulation of inflammatory responses in ferrets (Mustela putorius furo). CCL8 is a member of the CC chemokine family, which includes related chemokines such as CCL2 (MCP-1), CCL7 (MCP-3), and CCL13 (MCP-4) that coordinate monocyte and leukocyte trafficking during immune activation. In ferrets, CCL8 is produced by macrophages, fibroblasts, endothelial cells, epithelial cells, and other immune cells in response to infection, inflammatory cytokines, or tissue injury. CCL8 exerts its biological effects through interaction with chemokine receptors including CCR1, CCR2, CCR3, and CCR5, promoting chemotaxis and activation of monocytes, macrophages, dendritic cells, T lymphocytes, and eosinophils at sites of infection or inflammation. Ferrets are widely used as animal models for respiratory viral infections, particularly influenza and coronaviruses, and chemokines such as CCL8 are important for recruiting immune cells to the respiratory tract and regulating inflammatory responses during viral infection. Consequently, ferret CCL8 is studied in infectious disease, immunology, and vaccine research, helping to elucidate mechanisms of chemokine-mediated immune cell trafficking and host responses to respiratory pathogens.

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