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Rat Erythropoietin (EPO) Recombinant Protein
- Catalog Number:
- RP1805R
- Availability:
- In stock
- Application:
- Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat), Rattus rattus (black rat)
Powered by Bioz - Rat Erythropoietin (EPO) (catalog RP1805R) is a yeast-derived glycoprotein hormone supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.5 kDa, 166 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat and black rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study erythropoietin signaling and hematopoiesis (including regulation of red blood cell production, erythroid progenitor cell proliferation, and differentiation); typical experimental uses include cell-culture stimulation and proliferation assays, hematopoietic and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APPRLICDSR VLERYILEAK EAENVTMGCA EGPRLSENIT VPDTKVNFYA WKRMKVEEQA VEVWQGLSLL SEAILQAQAL QANSSQPPES LQLHIDKAIS GLRSLTSLLR VLGAQKELMS PPDATQAAPL RTLTADTFCK LFRVYSNFLR GKLKLYTGEA CRRGDR (166) |
| Endotoxin | Naturally endotoxin-free |
| Form | Lyophilized |
| Storage Conditions | -20°C |
| Molecular Weight | 18.5 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Expression System | Yeast |
| Form | Lyophilized |
| Country Of Origin | USA |
Rat erythropoietin (EPO) is a glycoprotein hormone produced primarily by the kidneys in response to hypoxia that regulates erythropoiesis by binding to the erythropoietin receptor (EPOR) on erythroid progenitor cells, activating JAK2/STAT5, PI3K/Akt, and MAPK signaling pathways to promote red blood cell survival, proliferation, and differentiation. In rats, EPO is essential for maintaining oxygen homeostasis and is widely studied in models of anemia, chronic kidney disease, ischemia, and hypoxia. Beyond its hematopoietic function, rat EPO has demonstrated tissue-protective effects in experimental models of stroke, myocardial infarction, spinal cord injury, and acute kidney injury through anti-apoptotic and anti-inflammatory mechanisms. As one of the most extensively characterized laboratory species, the rat is a valuable animal model for investigating erythropoiesis, hypoxia-inducible factor (HIF) signaling, renal physiology, and the preclinical development of erythropoiesis-stimulating agents and other therapies for anemia and tissue injury relevant to human medicine.
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