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Bovine Regakine-1 Recombinant Protein
- Catalog Number:
- RP1823B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos indicus (zebu), Bos taurus (cattle)
- Bovine Regakine-1 (catalog RP1823B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.3 kDa, 73 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle and zebu. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study Regakine-1 signaling and immune responses (including chemotactic activity and recruitment of leukocytes during inflammatory processes); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | EANEEPAGNM RVCCFSSVTR KIPLSLVKNY ERTGDKCPQE AVIFQTRSGR SICANPGQAW VQKYIEYLDQ MSK (73) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.3 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine Regakine-1 (also known as CCL28, mucosae-associated epithelial chemokine, or MEC) is a member of the CC chemokine family, a group of small secreted proteins that regulate leukocyte trafficking and mucosal immune responses. In cattle (Bos taurus), Regakine-1/CCL28 is primarily produced by epithelial cells of mucosal tissues, including the mammary gland, respiratory tract, and gastrointestinal tract, where it contributes to immune cell recruitment and mucosal immunity. This chemokine signals mainly through the CCR10 receptor, and to a lesser extent CCR3, promoting chemotaxis of IgA-secreting plasma cells, T lymphocytes, and other immune cells to mucosal surfaces. In bovine physiology, Regakine-1 plays an important role in mammary gland immune defense, particularly during lactation, where it helps recruit IgA-producing cells to the mammary epithelium, supporting antibody secretion into milk and enhancing protection of the mammary gland and neonate against pathogens. Regakine-1 is also involved in immune responses to mucosal infections and inflammatory conditions, including mastitis, where chemokine-mediated leukocyte recruitment contributes to host defense. Because of its role in mucosal immunity, antibody-secreting cell trafficking, and epithelial immune signaling, bovine Regakine-1 is studied in research on mammary gland immunity, mucosal immune regulation, and host-pathogen interactions relevant to cattle health and dairy production.
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