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Bovine Midkine Recombinant Protein
- Catalog Number:
- RP1832B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos taurus (cattle)
- Bovine Midkine (MK) (catalog RP1832B) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.4 kDa, 122 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle (with noted substitution [P107S]). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study midkine signaling and cellular processes (including regulation of cell growth, survival, migration, and development); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AKKKDKMKKG GPGSECAEWT WGPCTPSSKD CGVGFREGTC GAQTQRIRCR VPCNWKKEFG ADCKYKFETW GACDGGTGTK ARQGTLKKAR YNAQCQETIR VTKPYSSKTK AKAKAKKGKE KD (122) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.4 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine Midkine (MK) is a heparin-binding growth factor and cytokine that plays important roles in cell growth, survival, and tissue repair in cattle (Bos taurus). Midkine is expressed during embryonic development and in certain adult tissues, particularly in response to injury, inflammation, or stress. Bovine midkine interacts with several cell surface receptors, including receptor-type protein tyrosine phosphatase ζ (PTPRZ1), low-density lipoprotein receptor-related protein (LRP), and integrins, activating intracellular signaling pathways such as MAPK, PI3K-AKT, and NF-κB that regulate cell proliferation, migration, and survival. Through these mechanisms, midkine contributes to processes including tissue regeneration, angiogenesis, immune cell recruitment, and inflammatory responses. In cattle, midkine is associated with developmental processes, tissue repair, and immune regulation in response to injury or infection. In cell culture, recombinant bovine midkine is commonly used to stimulate cell proliferation and migration, study growth factor signaling pathways, and investigate mechanisms of tissue repair and inflammatory signaling in vitro. Bovine midkine is also studied in veterinary and comparative biomedical research to better understand growth factor-mediated regulation of development, inflammation, and tissue regeneration.
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