Slide 1 of 1 
Canine Amphiregulin Recombinant Protein
- Catalog Number:
- RP1846D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine Amphiregulin (AR) (catalog RP1846D) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~10.1 kDa, 87 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study amphiregulin/EGFR signaling and cellular processes (including regulation of cell proliferation, differentiation, tissue repair, and epithelial biology); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SVRVEQVVKP MKNKTESEKT SDKPKRKKKG GKSGKNRRNR KKKNPCDAEF QNFCIHGECK YIEHLEAVTC QCHQDYFGER CGEKSMK (87) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 10.1 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and functions as a ligand for the epidermal growth factor receptor (EGFR/ErbB1), where it regulates epithelial cell proliferation, differentiation, survival, and tissue repair. In dogs (Canis lupus familiaris), amphiregulin is produced by epithelial cells, keratinocytes, fibroblasts, airway smooth muscle cells, and activated immune cells-including Th2 cells and regulatory T cells-in response to tissue injury, inflammation, and allergic stimulation. Through activation of EGFR-dependent signaling pathways such as MAPK and PI3K/AKT, canine amphiregulin contributes to wound healing, mucosal barrier integrity, and tissue remodeling in cutaneous, respiratory, gastrointestinal, and mammary tissues. Dysregulated amphiregulin expression may be associated with chronic inflammatory diseases such as atopic dermatitis and allergic airway disease, fibrotic processes, and neoplastic conditions including mammary carcinoma, squamous cell carcinoma, and other EGFR-driven tumors. In veterinary and comparative oncology research, canine amphiregulin is particularly relevant due to the use of naturally occurring canine cancers and inflammatory diseases as translational models for human EGFR-targeted therapies. Characterizing amphiregulin expression in dogs provides valuable insight into epithelial-immune interactions, tissue repair mechanisms, and EGFR-mediated signaling in both veterinary medicine and comparative biomedical research.
Please choose a size to see price
RELATED MEDIA
