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Bovine Amphiregulin Recombinant Protein
- Catalog Number:
- RP1857B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bison bison bison (American buffalo), Bos indicus (zebu), Bos taurus (cattle)
- Bovine Amphiregulin (AR) (catalog RP1857B) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~10.2 kDa, 87 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, American buffalo, and zebu. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study amphiregulin/EGFR signaling and cellular processes (including regulation of cell proliferation, differentiation, tissue repair, and epithelial biology); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SVRVEQVVKP KKNKTESEKT SDKPKRKKKG GKNGKNRRNR KKKNLCDTEF QNFCIHGKCT FLEQLETVSC QCYPEYFGER CGEKSMK (87) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 10.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and functions as a ligand for the epidermal growth factor receptor (EGFR/ErbB1), where it regulates epithelial cell proliferation, differentiation, survival, and tissue repair. In cattle (Bos taurus), amphiregulin is produced by epithelial cells, keratinocytes, fibroblasts, mammary epithelial cells, and activated immune cells in response to tissue injury, inflammatory cytokines, and microbial stimulation. Through activation of EGFR-dependent signaling pathways such as MAPK and PI3K/AKT, bovine amphiregulin contributes to mucosal barrier maintenance, wound healing, mammary gland development and remodeling, and regulation of inflammatory responses in respiratory, gastrointestinal, cutaneous, and mammary tissues. Dysregulated amphiregulin expression may be associated with chronic inflammation, fibrosis, mastitis-related tissue remodeling, and neoplastic processes. In veterinary and agricultural research, bovine amphiregulin is of particular interest in studies of mastitis, mammary gland biology and lactation, respiratory disease, wound repair, and comparative models of EGFR-mediated signaling. Characterizing amphiregulin expression in cattle provides valuable insight into epithelial-immune crosstalk, tissue regeneration, and inflammatory regulation in both livestock health and translational biomedical research contexts.
5 ug
Price: $170.00
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