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Bovine TIM-3 Recombinant Protein
- Catalog Number:
- RP1887B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos taurus (cattle)
- Bovine TIM-3 (T-cell Immunoglobulin and Mucin-domain containing-3) (catalog RP1887B) is a yeast-derived immune checkpoint protein supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.8 kDa, 169 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle (also highly homologous across zebu, yak, and water buffalo). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TIM-3 signaling and immune regulation (including roles in T-cell exhaustion, immune checkpoint pathways, and modulation of immune responses); typical experimental uses include cell-culture stimulation and receptor-binding assays, immune checkpoint and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AYVSQVGQNA DLPCTYSPAT TENLVPVCWG KGPCPVFECY SLVLRTDGRN VTYQTSSRYL LKRDLHKGDV TLTIKNVTLA DSGTYCCRIQ FPGLMNDRKS NLELIIKPAK VTPAWTPWRD ITTAFPRMLT TKGPVSETRT LKTLHDKNQT EISTLATELQ DMGATTRTG (169) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 18.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine TIM-3 (T cell immunoglobulin and mucin domain-containing protein 3, also known as HAVCR2, hepatitis A virus cellular receptor 2) is an immune checkpoint receptor that regulates T cell activation, immune tolerance, and inflammatory responses in cattle (Bos taurus). TIM-3 is a member of the TIM (T cell immunoglobulin and mucin domain) family of immune regulatory proteins, which includes TIM-1, TIM-3, and TIM-4, molecules that help control immune activation and maintain immune homeostasis. In cattle, TIM-3 is expressed on activated T lymphocytes, natural killer (NK) cells, dendritic cells, and macrophages, where it functions primarily as a negative regulator of immune responses. Engagement of TIM-3 with its ligands-such as galectin-9, phosphatidylserine, and HMGB1-delivers inhibitory signals that suppress T cell proliferation, cytokine production, and cytotoxic activity, thereby preventing excessive immune-mediated tissue damage. In bovine health, TIM-3 signaling has been associated with immune regulation during chronic infectious diseases, including bovine leukemia virus (BLV) infection, bovine tuberculosis caused by Mycobacterium bovis, and other persistent infections, where increased expression of immune checkpoint receptors can contribute to T cell exhaustion and reduced pathogen clearance. Because immune checkpoint pathways strongly influence host immunity and pathogen persistence, bovine TIM-3 is an important target of study in veterinary immunology, chronic infection research, and development of immunomodulatory strategies aimed at improving immune responses and disease resistance in cattle.
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