Rabbit TWEAK (TNFSF12) Recombinant Protein

Catalog Number:
RP1960U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit TWEAK (TNF-like Weak Inducer of Apoptosis) (catalog RP1960U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.7 kDa, 142 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TWEAK signaling and immune responses (including regulation of apoptosis, inflammation, tissue remodeling, and TNF-related pathways); typical experimental uses include cell-culture stimulation and apoptosis assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAAHYEVHPRP GQDGAQAGVD GTVSGWEEAK INSSNPLRYD RQIGKFIVTR AGLYYLYCQV HFDEGKAVYL KLDLLVDEVL ALRCLEEFSA TAASSLGPQL RLCQVSGLLP LRPGSSLRIR TLPWAHLKAA PFLTYFGLFQ VH (142)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight15.7 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit TWEAK (TNF-like Weak Inducer of Apoptosis, also known as TNFSF12) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), TRAIL, and RANKL that regulate immune signaling, apoptosis, and tissue remodeling. In rabbits (Oryctolagus cuniculus), TWEAK is produced primarily by immune cells such as macrophages, monocytes, dendritic cells, and T lymphocytes, and can also be expressed by endothelial cells and other tissue cells during inflammation or tissue injury. TWEAK exerts its biological effects mainly through binding to its receptor Fn14 (fibroblast growth factor–inducible 14, TNFRSF12A), activating intracellular signaling pathways including NF-κB and MAPK, which regulate cell survival, proliferation, apoptosis, cytokine production, and tissue remodeling. In rabbits, the TWEAK–Fn14 signaling axis is involved in inflammatory responses, immune regulation, and tissue repair following injury or infection. Because rabbits are widely used as experimental models for cardiovascular disease, immunology, and inflammatory research, characterization of rabbit TWEAK supports studies of TNF superfamily signaling, cytokine-mediated inflammation, and mechanisms of tissue injury and regeneration relevant to both veterinary and human biomedical research.

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Phone: 651-646-0089
Fax: 651-646-0095

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