Ferret IL-5 Recombinant Protein

Catalog Number:
RP1988FT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mustela lutreola (European mink), Mustela nigripes (black-footed ferret), Mustela putorius furo (domestic ferret)
  • Ferret IL-5 (Interleukin-5) (catalog RP1988FT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.1 kDa, 115 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic ferret, European mink, and black-footed ferret. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-5 signaling and immune responses (including regulation of eosinophil growth, differentiation, and activation, as well as roles in allergic inflammation); typical experimental uses include cell-culture stimulation and proliferation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceTPVEDPMNRL VAETLTLLST HRTLLIGDGN LMIPTPENKN HQLCIEEVFQ GIDILKNRTA QAAAVDKLFQ NLSFIKAHID SQKKKCGGER WRVKKFLDYL QVFLGVINTE WTTES (115)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.1 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret Interleukin-5 (IL-5) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and group 2 innate lymphoid cells in ferrets (Mustela putorius furo), where it plays a central role in eosinophil differentiation, activation, recruitment, and survival. IL-5 belongs to the common beta chain (βc) cytokine family, which also includes IL-3 and granulocyte–macrophage colony-stimulating factor (GM-CSF); these cytokines share the βc receptor subunit and are involved in regulating hematopoiesis and myeloid cell function. IL-5 signals through a receptor complex composed of IL-5Rα and the shared βc chain, activating JAK/STAT and MAPK pathways that promote eosinophil maturation in the bone marrow and enhance their effector functions in peripheral tissues. In ferret health and research, IL-5 is relevant in models of allergic airway inflammation and respiratory viral infections, where Th2 polarization and eosinophilic responses may influence airway pathology, mucus production, and tissue remodeling. While IL-5 supports protective immunity against parasitic infections, excessive or prolonged IL-5 expression can contribute to eosinophilic inflammation and lung injury. Because ferrets are widely used as translational models for human respiratory disease and vaccine studies, characterization of ferret IL-5 supports investigations into Th1/Th2 immune balance, eosinophil biology, and development of targeted anti–IL-5 therapeutic strategies relevant to allergic and airway inflammatory disorders.

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