Feline IL-36 Receptor Antagonist (IL-36RA) Recombinant Protein

Catalog Number:
RP1989F
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Acinonyx jubatus (cheetah), Felis catus (domestic cat), Leopardus geoffroyi (Geoffroy's cat), Lynx pardinus (Spanish lynx), Lynx rufus (bobcat), Panthera leo (lion), Panthera pardus (leopard), Panthera tigris (tiger), Panthera uncia (snow leopard), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma)
  • Feline IL-36RA (Interleukin-36 Receptor Antagonist) (catalog RP1989F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.9 kDa, 154 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, tiger, leopard, cheetah, lion, leopard cat, Geoffroy's cat, bobcat, fishing cat, snow leopard, Spanish lynx, and puma. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-36RA signaling and immune responses (including inhibition of IL-36-mediated inflammatory pathways and regulation of epithelial and immune responses); typical experimental uses include cell-culture stimulation and cytokine modulation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVLSGALCFRM KDAELKVLYL HDNQLLAGRL HAGNIIQGEE ISVVPNRSLD AKLSPVILGV QGGSQCLSCG TGQEPTLKLE PVNIMELYLG AKESKSFTFY RRDTGLTSSF ESAAYPGWFL CTVPEADQPL RLTQLSEDAS WDSPITDFYF QQCD (154)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Feline Interleukin-36 Receptor Antagonist (IL-36Ra) is an anti-inflammatory cytokine that regulates IL-36–mediated immune responses in cats (Felis catus). IL-36Ra is a member of the IL-1 cytokine family and functions as a natural inhibitor of the IL-36 signaling pathway by binding to the IL-36 receptor (IL-36R, also known as IL1RL2) without inducing receptor activation, thereby preventing proinflammatory cytokines such as IL-36α, IL-36β, and IL-36γ from initiating downstream signaling. Through this mechanism, feline IL-36Ra suppresses activation of intracellular pathways including NF-κB and MAPK, helping to limit inflammatory mediator production and regulate immune responses at epithelial barrier tissues such as the skin and mucosal surfaces. In cats, IL-36Ra contributes to maintaining immune homeostasis and controlling excessive inflammatory responses. In cell culture, recombinant feline IL-36Ra is commonly used to inhibit IL-36–induced signaling in epithelial and immune cells, allowing researchers to study regulation of inflammatory pathways, cytokine production, and epithelial–immune cell interactions in vitro. Feline IL-36Ra is also relevant in translational medicine research because dysregulation of IL-36 signaling is associated with inflammatory skin diseases in humans, including generalized pustular psoriasis; feline and other animal models help researchers investigate IL-36 pathway regulation and evaluate potential anti-inflammatory therapeutic strategies targeting IL-36 signaling.

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