Feline CCL8 (MCP-2) Recombinant Protein

Catalog Number:
RP2016F
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Acinonyx jubatus (cheetah), Felis catus (domestic cat), Lynx canadensis (Canada lynx), Lynx rufus (bobcat), Panthera leo (lion), Panthera pardus (leopard), Panthera uncia (snow leopard), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma), Puma yagouaroundi (jaguarundi)
  • Feline CCL8 (C-C Motif Chemokine Ligand 8) (catalog RP2016F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~8.7 kDa, 76 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, cheetah, Canada lynx, bobcat, lion, leopard, snow leopard, leopard cat, fishing cat, puma, and jaguarundi. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, including leukocyte recruitment, migration, inflammation, and cytokine signaling; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQPGSVSIPIT CCFSVVKGKI PMQKLESYTR ITNIQCPQEA VIFKTKTSRE ICADPKQKWV QDFMKRLDQK SQTQKP (76)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.7kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Feline CCL8 (C–C motif chemokine ligand 8, also known as MCP-2 – Monocyte Chemoattractant Protein-2) is a proinflammatory chemokine that regulates leukocyte recruitment and inflammatory responses in cats (Felis catus). CCL8 belongs to the CC chemokine family, which includes related chemokines such as CCL2 (MCP-1), CCL7 (MCP-3), and CCL13 (MCP-4) that coordinate monocyte and leukocyte trafficking during immune activation. In cats, CCL8 is produced by macrophages, fibroblasts, endothelial cells, epithelial cells, and other immune cells in response to infection, inflammatory cytokines, or tissue injury. CCL8 exerts its biological effects through interaction with chemokine receptors including CCR1, CCR2, CCR3, and CCR5, promoting chemotaxis and activation of monocytes, macrophages, dendritic cells, T lymphocytes, and eosinophils at sites of infection or inflammation. In feline health, CCL8 likely contributes to immune responses against viral and bacterial pathogens, including infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), where chemokine-mediated recruitment of immune cells helps coordinate antiviral immunity. Because chemokines play critical roles in immune cell trafficking and inflammatory regulation, feline CCL8 is studied in veterinary immunology, infectious disease research, and inflammatory disease models to better understand immune responses and disease mechanisms in cats.

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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