Mouse CXCL1 (GRO alpha) Recombinant Protein

Catalog Number:
RP2019M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus musculus (house mouse)
  • Mouse CXCL1 (C-X-C Motif Chemokine Ligand 1) (catalog RP2019M) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.4 kDa, 68 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly neutrophil recruitment, inflammation, and cytokine signaling; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceNELRCQCLQT MAGIHLKNIQ SLKVLPSGPH CTQTEVIATL KNGREACLDP EAPLVQKIVQ KMLKGVPK (68)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.4kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse CXCL1 (C-X-C motif chemokine ligand 1), commonly known as KC (keratinocyte-derived chemokine), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL2 (MIP-2), CXCL3, and CXCL8 (IL-8) that regulate neutrophil recruitment during inflammatory responses. In mice (Mus musculus), CXCL1 is produced by macrophages, epithelial cells, endothelial cells, fibroblasts, and keratinocytes in response to infection, inflammatory cytokines, or tissue injury. CXCL1 primarily signals through the chemokine receptor CXCR2, promoting chemotaxis, activation, and migration of neutrophils and other innate immune cells to sites of infection or inflammation. In murine physiology, CXCL1 plays a critical role in innate immune defense, particularly during bacterial infections and inflammatory responses in tissues such as the lung, skin, and gastrointestinal tract. Mouse CXCL1 has been extensively studied in experimental animal models of inflammatory disease, including lipopolysaccharide (LPS)-induced acute lung injury, where CXCL1-mediated neutrophil recruitment drives pulmonary inflammation and tissue damage. Because mice are widely used as genetic and experimental models for immunology and inflammatory diseases, mouse CXCL1 has been central to understanding neutrophil trafficking, chemokine signaling pathways, and mechanisms of acute and chronic inflammation relevant to human disease.

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