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Bovine CCL14 Recombinant Protein
- Catalog Number:
- RP2051B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos indicus (zebu), Bos taurus (cattle)
- Bovine CCL14 (C-C Motif Chemokine Ligand 14) (catalog RP2051B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~8.3 kDa, 73 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle and zebu. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, including leukocyte recruitment, inflammation, and cytokine signaling; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SKNGSSSRGP YHPAECCLTY VSRPVPRQRV SSYYETSSQC PKPGIIFITK KGHYICANPR DGWVQDYIKE LEE (73) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.3 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine CCL14 (C-C motif chemokine ligand 14), also known as HCC-1 (Hemofiltrate CC chemokine-1), is a member of the CC chemokine family that participates in leukocyte trafficking and regulation of inflammatory responses in cattle (Bos taurus). CCL14 is produced primarily by monocytes, macrophages, epithelial cells, and certain stromal tissues, and-following proteolytic activation-functions through chemokine receptors such as CCR1, CCR3, and CCR5 to promote chemotaxis of monocytes, eosinophils, and specific T lymphocyte subsets. Similar to other species, bovine CCL14 is synthesized as a precursor molecule that requires processing to achieve full biological activity. In healthy cattle, basal expression is generally low but detectable, contributing to immune surveillance; however, expression may increase during mastitis, respiratory disease, enteric infection, parasitic challenge, or other inflammatory conditions. In veterinary and agricultural research, bovine CCL14 is of interest as a mediator of mononuclear and eosinophilic cell recruitment and as a biomarker of inflammatory activation and host-pathogen interactions in cattle.
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