Mink IL-8 Recombinant Protein

Catalog Number:
RP2052MK
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Neogale vison (American mink)
  • Mink IL-8 (Interleukin-8, CXCL8) (catalog RP2052MK) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~9.0 kDa, 79 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to American mink. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly neutrophil recruitment, inflammation, and cytokine signaling; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLVN GKEVCLDPKE DWVQRIVQIF LKKAEKQAS (79)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight9.0kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mink Interleukin-8 (IL-8), also known as CXCL8, is a pro-inflammatory chemokine that plays a central role in innate immune responses in mink (Neovison vison) by mediating neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, epithelial cells, and endothelial cells in response to viral, bacterial, and inflammatory stimuli, mink IL-8 signals primarily through CXCR1 and CXCR2 receptors to promote rapid recruitment of neutrophils and amplification of local inflammatory responses. IL-8 is particularly relevant in mink health due to its involvement in diseases such as Aleutian disease virus (ADV) infection, mink viral enteritis, bacterial pneumonia, and SARS-CoV-2 infection, where dysregulated inflammatory and chemokine responses can contribute to tissue damage and disease severity. Because mink have served as important natural and experimental models for SARS-CoV-2 transmission, influenza, and immune complex–mediated diseases such as Aleutian disease, characterization of IL-8 is critical for understanding neutrophil-driven pathology, cytokine and chemokine cascades, and host responses to vaccination or antiviral interventions. As both a biomarker and mediator of inflammation, mink IL-8 supports translational research in viral pathogenesis, immune dysregulation, and comparative immunology relevant to both animal health and human infectious disease studies.

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