Feline CXCL9 (MIG) Recombinant Protein

Catalog Number:
RP2078F
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Felis catus (domestic cat)
  • Feline CXCL9 (C-X-C Motif Chemokine Ligand 9) (catalog RP2078F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~12.0 kDa, 104 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for at least ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly T-cell recruitment, inflammation, and interferon-γ-induced signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceTLIMRNRRCF CINTSQGTIH LKSLKDLKQF APSPSCEKTE IIVTMKNGDQ TCLNPDSADV QELIKEWEKQ VTKRKSKKKG KKNQKSRKIF KVKKSQHPHQ KKTT (104)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight12.0kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Feline CXCL9 (C-X-C motif chemokine ligand 9), also known as MIG (monokine induced by interferon-γ), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL10 (IP-10) and CXCL11 (I-TAC) that regulate T-cell recruitment during interferon-driven immune responses. In cats (Felis catus), CXCL9 is produced by macrophages, dendritic cells, endothelial cells, and epithelial cells in response to interferon-γ (IFN-γ) and other inflammatory cytokines during infection or immune activation. CXCL9 primarily signals through the chemokine receptor CXCR3, which is expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and accumulation of these cells at sites of infection or inflammation. In feline health, CXCL9 contributes to cell-mediated immune responses against intracellular pathogens, including viral and protozoal infections, and plays a role in inflammatory processes affecting tissues such as the lung, skin, and lymphoid organs. Cats are also used as animal models for human viral and immunological diseases, particularly feline immunodeficiency virus (FIV) infection, which serves as a model for human HIV infection, where CXCL9 is part of the interferon-regulated chemokine network that directs T-cell trafficking and antiviral immune responses.

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