Human CXCL1 (GRO alpha) Recombinant Protein

Catalog Number:
RP2082H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan troglodytes (chimpanzee)
  • Human CXCL1 (C-X-C Motif Chemokine Ligand 1) (catalog RP2082H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~10.2 kDa, 70 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, western lowland gorilla, and chimpanzee. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly neutrophil recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceATELRCQCLQ TLQGIHPKNI QSVNVKSPGP HCAQTEVIAT LKNGRKACLN PASPIVKKII EKMLNSDKSN (70)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight10.2kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human CXCL1 (C-X-C motif chemokine ligand 1), also known as GRO-α (growth-regulated oncogene alpha), is a proinflammatory chemokine belonging to the CXC chemokine family, which includes related chemokines such as CXCL2 (GRO-β), CXCL3 (GRO-γ), and CXCL8 (IL-8) that regulate neutrophil recruitment during inflammatory responses. In humans (Homo sapiens), CXCL1 is produced by macrophages, epithelial cells, endothelial cells, fibroblasts, and tumor cells in response to infection, inflammatory cytokines, or tissue injury. CXCL1 primarily signals through the chemokine receptor CXCR2, promoting chemotaxis, activation, and migration of neutrophils and other innate immune cells to sites of infection or inflammation. Through these actions, CXCL1 plays an important role in host defense against bacterial infections and regulation of acute inflammatory responses, particularly in tissues such as the lung, skin, and gastrointestinal tract. Dysregulated CXCL1 expression has also been implicated in chronic inflammatory diseases, cancer progression, and tumor-associated inflammation, where CXCL1-mediated neutrophil recruitment and angiogenic signaling can influence the tumor microenvironment and disease progression. Because of its role in neutrophil trafficking and inflammatory signaling, human CXCL1 is widely studied in immunology, cancer biology, and inflammatory disease research.

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