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Bovine Osteopontin (OPN) Recombinant Protein
- Catalog Number:
- RP2089B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bison bison bison (American buffalo), Bos grunniens (domestic yak), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)
- Bovine OPN (Osteopontin) (catalog RP2089B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~29.2 kDa, 262 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, American buffalo, zebu, domestic yak, and wild yak. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study osteopontin-mediated signaling and biological processes, including cell adhesion, migration, inflammation, and immune regulation; typical applications include cell-based assays, cytokine and extracellular matrix signaling studies, inflammation research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | LPVKPTSSGS SEEKQLNNKY PDAVATWLKP DPSQKQTFLA PQNSVSSEET DDNKQNTLPS KSNESPEQTD DLDDDDDNSQ DVNSNDSDDA ETTDDPDHSD ESHHSDESDE VDFPTDIPTI AVFTPFIPTE SANDGRGDSV AYGLKSRSKK FRRSNVQSPD ATEEDFTSHI ESEEMHDAPK KTSQLTDHSK ETNSSELSKE LTPKAKDKNK HSNLIESQEN SKLSQEFHSL EDKLDLDHKS EEDKHLKIRI SHELDSASSE VN (262) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 29.2kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine Osteopontin (OPN) (also known as SPP1, Secreted Phosphoprotein 1, bone sialoprotein I, ETA-1, or early T-lymphocyte activation-1) is a multifunctional phosphorylated glycoprotein involved in immune regulation, cell adhesion, and tissue remodeling in cattle (Bos taurus). Osteopontin interacts with integrins (such as αvβ3 and αvβ1) and CD44 receptors, promoting signaling pathways that regulate cell migration, inflammation, and extracellular matrix remodeling. In cattle, OPN is produced by a variety of cells including osteoblasts, macrophages, epithelial cells, and activated T lymphocytes, and plays important roles in bone remodeling, immune responses, and reproductive biology. In bovine health, osteopontin is highly expressed in the mammary gland and uterine endometrium, where it contributes to embryo implantation, placental development, and early pregnancy maintenance, making it an important molecule in bovine fertility and reproductive success. OPN is also involved in inflammatory responses during mastitis and other infections, where it helps regulate leukocyte recruitment and cytokine signaling. While osteopontin supports tissue repair and immune defense, excessive expression may contribute to chronic inflammation or fibrotic tissue remodeling. As a regulator of immune cell migration, tissue remodeling, and reproductive signaling, bovine osteopontin is widely studied in host-pathogen interactions, mammary gland immunity, and reproductive physiology, contributing to improved understanding of cattle health, fertility, and productivity.
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