Human CCL8 (MCP-2) Recombinant Protein

Catalog Number:
RP2090H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Homo sapiens (human), Pongo abelii (Sumatran orangutan)
  • Human CCL8 (C-C Motif Chemokine Ligand 8) (catalog RP2090H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~8.9 kDa, 76 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human and Sumatran orangutan. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, including leukocyte recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP (76)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.9kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human CCL8 (C–C motif chemokine ligand 8, also known as MCP-2 – Monocyte Chemoattractant Protein-2) is a proinflammatory chemokine that regulates leukocyte recruitment and inflammatory responses in humans (Homo sapiens). CCL8 is a member of the CC chemokine family, which includes related chemokines such as CCL2 (MCP-1), CCL7 (MCP-3), and CCL13 (MCP-4) that coordinate monocyte and leukocyte trafficking during immune activation. In humans, CCL8 is produced by macrophages, fibroblasts, endothelial cells, epithelial cells, and other immune cells in response to infection, inflammatory cytokines, or tissue injury. CCL8 exerts its biological effects through interaction with chemokine receptors including CCR1, CCR2, CCR3, and CCR5, promoting chemotaxis and activation of monocytes, macrophages, dendritic cells, T lymphocytes, eosinophils, and basophils at sites of inflammation. Through these actions, CCL8 contributes to host defense against viral, bacterial, and parasitic pathogens and helps coordinate innate and adaptive immune responses. Dysregulated CCL8 expression has also been associated with chronic inflammatory diseases, allergic disorders, and cancer, where it can influence immune cell infiltration and tumor microenvironment signaling. Because of its central role in chemokine-mediated leukocyte trafficking and immune regulation, human CCL8 is widely studied in immunology, infectious disease, cancer biology, and inflammatory disease research.

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