Hamster CXCL12 (SDF-1) Recombinant Protein

Catalog Number:
RP2092J
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mesocricetus auratus (golden hamster)
  • Hamster CXCL12 (C-X-C Motif Chemokine Ligand 12) (catalog RP2092J) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.9 kDa, 68 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to golden hamster. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated signaling, particularly cell migration, stem cell homing, immune cell trafficking, and CXCR4/CXCR7 signaling pathways; typical applications include chemotaxis and migration assays, stem cell and cancer research, cytokine signaling studies, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKSNNRQV CIDPKLKWIQ EYLEKALNKR FKM (73)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.9kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Hamster CXCL12 (C-X-C motif chemokine ligand 12), also known as SDF-1 (stromal cell–derived factor-1), is a homeostatic chemokine belonging to the CXC chemokine family that plays a central role in cell migration, immune cell trafficking, and tissue development. In hamsters (commonly Syrian or golden hamster, Mesocricetus auratus), CXCL12 is produced by stromal cells, endothelial cells, epithelial cells, fibroblasts, and bone marrow niche cells, where it helps regulate the migration and positioning of hematopoietic stem cells, lymphocytes, and other progenitor cells. CXCL12 primarily signals through the receptors CXCR4 and ACKR3 (CXCR7), guiding immune cells and stem cells to specific tissues and supporting hematopoiesis, immune surveillance, and tissue repair. In hamster research models, the CXCL12–CXCR4 signaling axis has been examined in studies of viral infection and tissue inflammation, particularly in Syrian hamster models of SARS-CoV-2 infection, where chemokine-driven immune cell recruitment contributes to lung inflammation and antiviral immune responses. Because hamsters replicate several aspects of human respiratory viral disease, characterization of CXCL12 in this species helps researchers investigate immune cell trafficking, inflammatory signaling, and tissue repair mechanisms in infectious disease and immunology research.

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