Multi-species (Jamaican Fruit-eating Bat, Common Vampire Bat) CCL5 (RANTES) Recombinant Protein

Catalog Number:
RP2108BT
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Artibeus jamaicensis (Jamaican fruit-eating bat), Desmodus rotundus (common vampire bat)
  • Bat CCL5 (C-C Motif Chemokine Ligand 5) (catalog RP2108BT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.9 kDa, 68 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Jamaican fruit-eating bat and common vampire bat. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly leukocyte recruitment, inflammation, and antiviral immune responses; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPYASDTTPC CFAYISGRLP FTHVQEYFYT SSKCSMPAVV FVTRKHRQVC ANPQKKWVRE YINSLEMS (68)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.9kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids68
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Jamaican Fruit-eating Bat (Artibeus jamaicensis) and Common Vampire Bat (Desmodus rotundus) CCL5 (C–C motif chemokine ligand 5, also known as RANTES – Regulated upon Activation, Normal T cell Expressed and Secreted) is a proinflammatory chemokine involved in immune cell recruitment and antiviral immune responses. CCL5 belongs to the CC chemokine family, which includes related chemokines such as CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL2 (MCP-1) that regulate leukocyte trafficking during immune activation. In both Jamaican Fruit-eating bats and Common Vampire bats, the CCL5 protein is identical at the amino acid level, indicating strong evolutionary conservation and suggesting similar biological functions in these two bat species. CCL5 is produced primarily by activated T lymphocytes, macrophages, dendritic cells, platelets, and epithelial cells in response to viral infection, inflammatory stimuli, or tissue injury. It exerts its biological effects mainly through binding to the chemokine receptors CCR1, CCR3, and CCR5, which activates intracellular signaling pathways that promote chemotaxis and activation of T cells, monocytes, macrophages, eosinophils, and natural killer (NK) cells at sites of infection or inflammation. In bats, chemokines such as CCL5 likely contribute to antiviral defense and coordination of innate and adaptive immunity, particularly during infections with viruses that many bat species naturally harbor. Because Jamaican Fruit-eating bats and Common Vampire bats are recognized reservoirs for multiple emerging viruses, characterization of CCL5 helps support research into bat immune regulation, chemokine-mediated leukocyte recruitment, and host–pathogen interactions that enable bats to tolerate viral infections while limiting excessive inflammatory damage.
  • Chemokine

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