Cynomolgus Monkey EGF Recombinant Protein

Catalog Number:
RP2134Y
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Macaca fascicularis (crab-eating macaque), Macaca nemestrina (pig-tailed macaque), Pongo abelii (Sumatran orangutan), Pongo pygmaeus (Bornean orangutan), Rhinopithecus roxellana (golden snub-nosed monkey), Theropithecus gelada (gelada)
  • Cynomolgus Monkey EGF (Epidermal Growth Factor) (catalog RP2134Y) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~6.1 kDa, 53 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, pig-tailed macaque, Sumatran orangutan, Bornean orangutan, golden snub-nosed monkey, and gelada. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study EGF signaling and cellular processes, including cell proliferation, differentiation, wound healing, and receptor-mediated signaling pathways; typical applications include cell culture assays, growth and differentiation studies, signaling pathway research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceNSDSGCPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR (53)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight6.1kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids53
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In cynomolgus monkeys (Macaca fascicularis), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues such as salivary and gastrointestinal glands, where it plays important roles in epithelial maintenance, wound healing, and tissue regeneration. Binding of EGF to EGFR activates intracellular signaling pathways including MAPK/ERK, PI3K–AKT, and JAK/STAT, promoting cell proliferation, survival, and tissue remodeling following injury or inflammation. Because cynomolgus monkeys share strong genetic, physiological, and immunological similarities with humans, they are widely used as preclinical animal models for dermatology, gastrointestinal biology, and regenerative medicine. In these models, EGF signaling has been studied in nonhuman primate wound healing and tissue repair research, helping to evaluate EGFR-targeted therapies and growth factor–based treatments relevant to human skin regeneration and epithelial repair.
  • Growth Factor

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