Equine CXCL6 Recombinant Protein

Catalog Number:
RP2149E
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Equus asinus (ass), Equus caballus (horse), Equus przewalskii (Przewalski's horse), Equus quagga (plains zebra)
  • Equine CXCL6 (C-X-C Motif Chemokine Ligand 6) (catalog RP2149E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~8.3 kDa, 77 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse, Przewalski's horse, donkey, and plains zebra. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for at least ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly neutrophil recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGPVAAAVREL RCMCLTVTPG IHPKMISSLQ VFAVGPQCSK VEVVATLKNK KEVCLDPEAP LIKKFIQKTL DSGNKKN (77)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.3kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Equine CXCL6 (C-X-C motif chemokine ligand 6), also known as GCP-2 (granulocyte chemotactic protein 2), is a proinflammatory chemokine belonging to the ELR⁺ CXC chemokine family, which includes CXCL1, CXCL2, CXCL3, and CXCL5 that regulate neutrophil recruitment during inflammatory responses. In horses (Equus caballus), CXCL6 is produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to proinflammatory cytokines such as IL-1β and TNF-α, microbial infection, or tissue injury. CXCL6 primarily signals through the chemokine receptor CXCR2, which is expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. In equine health, CXCL6 contributes to innate immune responses in tissues such as the respiratory tract and skin, where neutrophil recruitment is important for controlling infections. CXCL6-mediated neutrophil trafficking is particularly relevant in equine respiratory diseases, including equine influenza and inflammatory airway disease, where chemokine-driven neutrophil accumulation influences airway inflammation, host defense, and disease severity. Consequently, equine CXCL6 is studied in veterinary immunology and respiratory disease research to better understand neutrophil-mediated inflammatory responses and host–pathogen interactions in horses.

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