Feline CCL4 (MIP-1 beta) Recombinant Protein

Catalog Number:
RP2205F
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Acinonyx jubatus (cheetah), Felis catus (domestic cat), Leopardus geoffroyi (Geoffroy's cat), Lynx canadensis (Canada lynx), Lynx pardinus (Spanish lynx), Lynx rufus (bobcat), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma), Puma yagouaroundi (jaguarundi)
  • Feline CCL4 (C-C Motif Chemokine Ligand 4) (catalog RP2205F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, cheetah, Geoffroy's cat, Canada lynx, Spanish lynx, bobcat, leopard cat, fishing cat, puma, and jaguarundi. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for at least ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly macrophage and lymphocyte recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPMGSDPPTA CCFSYALRKL PRNFVVDYFE TSSLCSQPAV VFQTKRGRQV CANPSDSWVQ EYMDDLELN (69)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Feline CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine that plays an important role in recruitment and activation of immune cells during inflammatory and antiviral immune responses in cats (Felis catus). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In cats, CCL4 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial products and cytokines including TNF-α and IL-1β. CCL4-mediated immune cell recruitment is particularly relevant in viral infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), where CCR5-associated chemokine signaling contributes to antiviral immune responses and regulation of leukocyte trafficking. It is also implicated in inflammatory conditions including feline infectious peritonitis (FIP), where macrophage-driven inflammation plays a central role in disease pathology. While CCL4-driven leukocyte migration supports pathogen clearance and immune activation, excessive or prolonged expression may contribute to inflammatory tissue damage. As a regulator of immune cell trafficking and inflammatory signaling, feline CCL4 is important for studies of host–pathogen interactions, viral immunity, and inflammatory disease mechanisms relevant to feline health and comparative immunology.

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Phone: 651-646-0089
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