Ferret CCL20 Recombinant Protein

Catalog Number:
RP2210FT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mustela lutreola (European mink), Mustela nigripes (black-footed ferret), Mustela putorius furo (domestic ferret)
  • Ferret CCL20 (C-C Motif Chemokine Ligand 20) (catalog RP2210FT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~8.1 kDa, 70 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to ferret, European mink, and black-footed ferret. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly lymphocyte and dendritic cell recruitment, mucosal immunity, and inflammatory signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceASNFDCCLRY TEHILHPKYI VGFTQQLANE ACDINAVIFY TKKKLAVCAD PKRNWVKKAV QILSHRVKKM (70)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.1kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret CCL20 (C-C motif chemokine ligand 20, also known as MIP-3α, macrophage inflammatory protein-3 alpha, or LARC – liver and activation-regulated chemokine) is a pro-inflammatory chemokine that plays an important role in mucosal immunity and leukocyte recruitment in ferrets (Mustela putorius furo). CCL20 signals primarily through the CCR6 receptor, promoting chemotaxis of immune cells such as immature dendritic cells, memory T cells, and certain B cell populations to sites of epithelial infection or inflammation. In ferret health and research, CCL20 is particularly relevant in respiratory mucosal immune responses, including models of influenza virus and SARS-CoV-2 infection, where epithelial cells in the airway produce CCL20 to recruit immune cells to infected lung tissue and initiate antiviral immune responses. CCL20-mediated signaling contributes to coordination of innate and adaptive immunity at mucosal surfaces but may also contribute to inflammatory lung pathology if excessively expressed. Because ferrets are widely used as translational models for human respiratory viral infections and mucosal immunity, characterization of ferret CCL20 supports studies of chemokine-driven immune cell trafficking, epithelial immune responses, and host–pathogen interactions relevant to human respiratory disease and vaccine development.

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