Multi-species (Ferret, Mink) PDGF-BB Recombinant Protein

Catalog Number:
RP2227FT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Enhydra lutris kenyoni (northern sea otter), Lontra canadensis (Northern American river otter), Lutra lutra (Eurasian river otter), Mustela lutreola (European mink), Mustela nigripes (black-footed ferret), Mustela putorius furo (domestic ferret), Neogale vison (American mink)
  • Ferret/Mink PDGF-BB (Platelet-Derived Growth Factor BB) (catalog RP2227FT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~18.3 kDa, 161 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to ferret, American mink, northern sea otter, northern American river otter, Eurasian river otter, European mink, and black-footed ferret. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study PDGF signaling and cellular processes, including cell proliferation, migration, angiogenesis, and tissue repair; typical applications include cell culture assays, growth and differentiation studies, signaling pathway research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSLGSPTVAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPTFKKATVT LEDHLACKCE TVVATRPVTR SPGSTQEQRA AKTPQTRVTI RTVRVRRPPK GKHRKFKHTH DKKALKETLG A (161)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight18.3kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ferret and Mink Platelet-Derived Growth Factor-BB (PDGF-BB) is a homodimeric growth factor composed of two PDGF-B chains that plays an important role in cell proliferation, migration, angiogenesis, and tissue repair in mustelids, including ferrets (Mustela putorius furo) and mink (Neovison vison), where the protein sequence is highly conserved and functionally identical. PDGF-BB signals primarily through platelet-derived growth factor receptor-β (PDGFR-β) and PDGFR-α/β receptor complexes, activating intracellular signaling pathways such as PI3K/AKT, MAPK, and STAT, which regulate fibroblast proliferation, smooth muscle cell growth, and extracellular matrix production. In ferrets and mink, PDGF-BB is produced by platelets, macrophages, endothelial cells, and fibroblasts and is released during tissue injury, inflammation, and wound healing. It plays important roles in vascular remodeling, dermal repair, and connective tissue regeneration, supporting recovery following injury or infection. PDGF-BB signaling may also contribute to fibrotic responses and tumor-associated stromal remodeling during chronic inflammation or neoplastic disease. Because ferrets and mink are used in comparative infectious disease and respiratory research, including models of influenza and SARS-CoV-2 infection, PDGF-BB is relevant to studies of tissue repair, inflammatory responses, and vascular changes that occur during infection and recovery.

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Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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