Dolphin IL-22 Recombinant Protein

Catalog Number:
RP2229P
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Delphinus delphis (saddleback dolphin), Tursiops truncatus (common bottlenose dolphin)
  • Dolphin IL-22 (Interleukin-22) (catalog RP2229P) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~17.8 kDa, 156 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to common bottlenose dolphin and saddleback dolphin. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study IL-22 signaling and immune responses, particularly in epithelial defense, inflammation, and cytokine-mediated signaling pathways; typical applications include cell-based assays, cytokine signaling studies, immunology research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVPIVSPCRLD RANFQQPYIT NRTFALANEA SLVDNNTDVR LIGTKLFQGV KMMERCCMLK QVLNFALEEV LLPQSNRFKP YMQEVVPFLA RLNKKLGQCH MECDNQNIQR NVQNLKDTVK KLGESGEIKV IGELNLLFMA LKNECTRPGQ SSEMDN (156)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight17.8kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Dolphin IL-22 (Interleukin-22) is a member of the IL-10 cytokine family that plays an important role in mucosal immunity and epithelial barrier protection in dolphins (e.g., Tursiops truncatus). IL-22 is produced primarily by activated CD4⁺ T cells, including Th17- and Th22-like populations, as well as by innate lymphoid cells in response to microbial infection and inflammatory stimulation. It signals through the IL-22 receptor complex (IL-22R1 and IL-10R2), expressed mainly on epithelial and stromal cells, activating downstream JAK/STAT pathways—particularly STAT3—to induce antimicrobial peptide production, enhance chemokine expression, promote epithelial cell proliferation, and support tissue repair. In healthy dolphins, basal IL-22 expression is expected to be low but increases during bacterial, viral, or fungal infections and during inflammatory challenges affecting respiratory, gastrointestinal, or dermal tissues. In marine mammal and comparative immunology research, dolphin IL-22 is of interest for understanding mucosal defense mechanisms, host responses to environmental stressors and pathogens, and the maintenance of epithelial integrity in both managed and free-ranging populations.

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