Bovine, Ovine, and Caprine CXCL3 (C-X-C motif chemokine ligand 3), also known as GRO-γ (growth-regulated oncogene gamma), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL1 (GRO-α) and CXCL2 (GRO-β) that regulate neutrophil recruitment during inflammatory responses. In cattle (Bos taurus), sheep (Ovis aries), and goats (Capra hircus), the CXCL3 protein is identical at the amino acid level, indicating strong evolutionary conservation and suggesting similar biological functions across these ruminant species. CXCL3 is produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to proinflammatory cytokines such as IL-1β and TNF-α, microbial infection, or tissue injury. It primarily signals through the chemokine receptor CXCR2, which is expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. In ruminant health, CXCL3 contributes to innate immune defense against bacterial and viral pathogens, particularly in conditions affecting the respiratory tract, skin, and mammary gland, where neutrophil recruitment is essential for pathogen clearance during diseases such as mastitis and respiratory infections. Because the protein sequence is identical across cattle, sheep, and goats, CXCL3 provides a useful molecule for comparative veterinary immunology studies, helping researchers understand neutrophil-mediated inflammation and chemokine-driven immune responses in ruminant livestock species.