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Multi-species (Bovine, Ovine, Caprine) CXCL3 Recombinant Protein
- Catalog Number:
- RP2233B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos javanicus (banteng), Bos taurus (cattle), Budorcas taxicolor (takin), Capra hircus (goat), Dama dama (fallow deer), Oryx dammah (scimitar-horned oryx), Ovis aries (sheep)
- Bovine/Ovine/Caprine CXCL3 (C-X-C Motif Chemokine Ligand 3) (catalog RP2233B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.9 kDa, 73 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, sheep, goat, banteng, takin, fallow deer, and scimitar-horned oryx. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly neutrophil recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APVVNELRCH CLQTLQGIHL KNIQSVKVTP PGPHCGQTEV IATLKNGQEA CLNPEAPMVK KIINKMLNKG STN (73) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.9kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine, Ovine, and Caprine CXCL3 (C-X-C motif chemokine ligand 3), also known as GRO-γ (growth-regulated oncogene gamma), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL1 (GRO-α) and CXCL2 (GRO-β) that regulate neutrophil recruitment during inflammatory responses. In cattle (Bos taurus), sheep (Ovis aries), and goats (Capra hircus), the CXCL3 protein is identical at the amino acid level, indicating strong evolutionary conservation and suggesting similar biological functions across these ruminant species. CXCL3 is produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to proinflammatory cytokines such as IL-1β and TNF-α, microbial infection, or tissue injury. It primarily signals through the chemokine receptor CXCR2, which is expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. In ruminant health, CXCL3 contributes to innate immune defense against bacterial and viral pathogens, particularly in conditions affecting the respiratory tract, skin, and mammary gland, where neutrophil recruitment is essential for pathogen clearance during diseases such as mastitis and respiratory infections. Because the protein sequence is identical across cattle, sheep, and goats, CXCL3 provides a useful molecule for comparative veterinary immunology studies, helping researchers understand neutrophil-mediated inflammation and chemokine-driven immune responses in ruminant livestock species.
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