Human CCL24 Recombinant Protein

Catalog Number:
RP2266H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Homo sapiens (human)
  • Human CCL24 (C-C Motif Chemokine Ligand 24) (catalog RP2266H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~10.5 kDa, 93 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly eosinophil recruitment, allergic inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVAVK GPVQRYPGNQ TTC (93)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight10.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human CCL24 (C-C motif chemokine ligand 24, also known as eotaxin-2 or MPIF-2, macrophage inflammatory protein-4) is a chemokine that plays an important role in recruitment of eosinophils and regulation of type 2 immune responses. CCL24 primarily signals through the CCR3 receptor, promoting chemotaxis of eosinophils, basophils, and Th2 lymphocytes to sites of infection, allergic inflammation, or tissue injury. In humans, CCL24 is produced by epithelial cells, macrophages, endothelial cells, and fibroblasts in response to Th2-associated cytokines such as IL-4 and IL-13. It is particularly active in mucosal and barrier tissues, including the lungs, gastrointestinal tract, and skin, where it contributes to immune defense against parasites but is also involved in allergic inflammation. Dysregulated or elevated CCL24 expression has been implicated in asthma, allergic rhinitis, atopic dermatitis, eosinophilic esophagitis, and other eosinophil-associated inflammatory diseases, where excessive eosinophil recruitment contributes to tissue inflammation and damage. CCL24 also participates in fibrotic disease processes, including pulmonary and liver fibrosis, through recruitment of inflammatory cells and modulation of tissue remodeling pathways. Because of its central role in eosinophil trafficking and Th2-mediated inflammation, human CCL24 is studied as both a biomarker and potential therapeutic target in allergic disease, chronic inflammation, and fibrotic disorders.

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