Mouse CCL22 Recombinant Protein

Catalog Number:
RP2267M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus musculus (house mouse)
  • Mouse CCL22 (C-C Motif Chemokine Ligand 22) (catalog RP2267M) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.8 kDa, 68 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly T-cell and dendritic cell recruitment, immune regulation, and inflammatory signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS (68)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse CCL22 (C-C motif chemokine ligand 22, also known as macrophage-derived chemokine, MDC) is an immunoregulatory chemokine that plays an important role in directing T cell trafficking and modulating immune responses in mice (Mus musculus). CCL22 primarily signals through the CCR4 receptor, promoting chemotaxis of Th2 cells, regulatory T cells (Tregs), and certain dendritic cell populations to sites of immune activation or inflammation. In mice, CCL22 is produced mainly by macrophages, dendritic cells, and epithelial cells following stimulation by inflammatory cytokines or pathogen-associated signals. CCL22-mediated CCR4 signaling contributes to regulation of immune balance by promoting recruitment of T cells involved in allergic responses, immune tolerance, and resolution of inflammation. In murine research, CCL22 is widely studied in experimental models of allergic airway disease (asthma), atopic dermatitis, autoimmune disorders, and tumor immunology, where it influences recruitment of regulatory T cells and Th2 cells to inflamed tissues or tumor microenvironments. While CCL22-driven immune cell trafficking helps regulate immune responses and maintain tolerance, excessive expression can contribute to chronic inflammation or immune suppression within tumors. Because mice are the primary genetic model for studying immune regulation, characterization of mouse CCL22 has been essential for understanding CCR4-mediated T cell migration, allergic inflammation, and tumor immune evasion, providing insights relevant to human immunology and therapeutic development.

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