Rabbit EGF Recombinant Protein

Catalog Number:
RP2300U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit EGF (Epidermal Growth Factor) (catalog RP2300U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~4.9 kDa, 47 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study EGF signaling and cellular processes, including cell proliferation, differentiation, wound healing, and receptor-mediated signaling pathways; typical applications include cell culture assays, growth and differentiation studies, signaling pathway research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceEGGYTCMCAG SVSEPGLICP DSVPPFQLKE DEPSPGRNSF PGCPPSH (47)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight4.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In rabbits (Oryctolagus cuniculus), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues such as salivary glands and gastrointestinal epithelium, where it supports epithelial maintenance, mucosal integrity, and wound healing. Binding of EGF to EGFR activates intracellular signaling pathways including MAPK/ERK, PI3K–AKT, and JAK/STAT, which promote cell proliferation, survival, and tissue regeneration following injury or inflammation. In rabbit physiology, EGF plays a key role in repair of skin and gastrointestinal epithelial tissues, helping restore barrier function after damage. Rabbits are also widely used as animal models for wound healing and ophthalmic research, and EGF signaling has been studied in rabbit models of corneal injury, where EGFR-mediated pathways regulate corneal epithelial regeneration and tissue repair, providing insights relevant to both veterinary medicine and human ocular therapeutics.

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