Multi-species (Ovine, Caprine) IL-36 Receptor Antagonist (IL-36RA) Recombinant Protein

Catalog Number:
RP2314V
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Capra hircus (goat), Ovis ammon polii (Marco Polo sheep), Ovis aries (sheep)
  • Ovine/Caprine IL-36RA (Interleukin-36 Receptor Antagonist) (catalog RP2314V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~17.1 kDa, 155 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat, sheep, and Marco Polo sheep. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study IL-36 signaling and immune regulation, particularly inhibition of IL-36-mediated inflammatory responses and cytokine activity; typical applications include cell-based assays, cytokine signaling studies, inflammation research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceMVLSGALCFR MKDAALKVLY LHDNQLLAGG LQAGKVIKGE EISVVPNRSL DAKLSPVILG VQGGSQCLSC GTGQEPTLKL EPVNIMELYH SAEKSKKFTF YRRDTGLTSS FESAAYPGWF LCTVPEADQP LQITQLPKDP SWDDPITDFY FQQCD (155)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight17.1 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Ovine/Caprine Interleukin-36 Receptor Antagonist (IL-36Ra) is an anti-inflammatory cytokine that regulates IL-36–mediated immune responses in sheep (Ovis aries) and goats (Capra hircus). The IL-36Ra protein is identical at the amino acid level in these two species, reflecting strong evolutionary conservation of this regulatory cytokine in small ruminants. IL-36Ra is a member of the IL-1 cytokine family and functions as a natural inhibitor of the IL-36 signaling pathway by binding to the IL-36 receptor (IL-36R, also known as IL1RL2) without activating it, thereby preventing proinflammatory cytokines such as IL-36α, IL-36β, and IL-36γ from initiating downstream signaling. Through this mechanism, ovine and caprine IL-36Ra suppress activation of intracellular pathways including NF-κB and MAPK, helping to limit production of inflammatory mediators and regulate immune responses at epithelial barrier tissues such as the skin, respiratory tract, and gastrointestinal mucosa. In sheep and goats, IL-36Ra contributes to maintaining immune homeostasis and controlling excessive inflammatory responses during infection or tissue injury. In cell culture, recombinant ovine/caprine IL-36Ra is commonly used to inhibit IL-36–induced inflammatory signaling in epithelial and immune cells, enabling investigation of cytokine regulation, epithelial–immune cell interactions, and modulation of inflammatory pathways in vitro.

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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