Human TNFSF15 Recombinant Protein

Catalog Number:
RP2321H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Homo sapiens (human)
  • Human TNFSF15 (Tumor Necrosis Factor Superfamily Member 15) (catalog RP2321H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~20.4 kDa, 180 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study TNFSF15 signaling and immune responses, particularly in inflammation, endothelial cell regulation, and cytokine-mediated signaling pathways; typical applications include cell-based assays, cytokine signaling studies, immunology research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceLKGQEFAPSH QQVYAPLRAD GDKPRAHLTV VRQTPTQHFK NQFPALHWEH ELGLAFTKNR MNYTNKFLLI PESGDYFIYS QVTFRGMTSE CSEIRQAGRP NKPDSITVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL (180)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight20.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human TNFSF15 (Tumor Necrosis Factor Superfamily Member 15, also known as TL1A or VEGI—vascular endothelial growth inhibitor) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), TRAIL, and RANKL that regulate immune signaling, apoptosis, and inflammatory processes. In humans, TNFSF15 is primarily produced by endothelial cells, macrophages, dendritic cells, and other immune cells in response to inflammatory stimuli, infection, or tissue injury. TNFSF15 exerts its biological effects mainly through binding to death receptor 3 (DR3, TNFRSF25) expressed on T lymphocytes, particularly Th1 and Th17 cells, activating intracellular signaling pathways including NF-κB and MAPK, which promote T cell activation, cytokine production, and inflammatory responses. In addition to its immune regulatory role, TNFSF15 also influences angiogenesis and vascular homeostasis, where it can inhibit endothelial cell proliferation and contribute to regulation of blood vessel formation. Dysregulation of TNFSF15 signaling has been implicated in several chronic inflammatory and autoimmune diseases, including inflammatory bowel disease (Crohn’s disease and ulcerative colitis), rheumatoid arthritis, and other immune-mediated disorders. Because TNFSF15 is an important regulator of T cell–mediated immunity and vascular biology, it is widely studied in immunology, inflammation research, and therapeutic development targeting TNF superfamily signaling pathways.

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