Feline TNFSF15 Recombinant Protein

Catalog Number:
RP2575F
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Acinonyx jubatus (cheetah), Felis catus (domestic cat), Leopardus geoffroyi (Geoffroy's cat), Lynx canadensis (Canada lynx), Lynx pardinus (Spanish lynx), Lynx rufus (bobcat), Neofelis nebulosa (Clouded leopard), Panthera leo (lion), Panthera pardus (leopard), Panthera tigris (tiger), Panthera uncia (snow leopard), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma), Puma yagouaroundi (jaguarundi)
  • Feline TNFSF15 (Tumor Necrosis Factor Superfamily Member 15) (catalog RP2575F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~20.2 kDa, 180 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, cheetah, Geoffroy's cat, Canada lynx, Spanish lynx, bobcat, clouded leopard, lion, leopard, tiger, snow leopard, leopard cat, fishing cat, puma, and jaguarundi. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study TNFSF15 signaling and immune responses, particularly in inflammation, endothelial cell regulation, and cytokine-mediated signaling pathways; typical applications include cell-based assays, cytokine signaling studies, immunology research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequencePKGREFGPSH QRAYTSPGAG GDKPRAHLTV VRQTPTQPLK NQFPALHWEH ELGLAFIKNR MNYTNKFLVI PESGDYFVYS QVTFRGTTSE CGEIRQGSRL NKPDSIIVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL (180)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight20.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Feline TNFSF15 (Tumor Necrosis Factor Superfamily Member 15, also known as TL1A or VEGI—vascular endothelial growth inhibitor) is a cytokine belonging to the tumor necrosis factor (TNF) superfamily, which includes related signaling molecules such as TNF-α, Fas ligand (FasL), CD40 ligand (CD40L), TRAIL, and RANKL that regulate immune responses, apoptosis, and inflammatory signaling. In cats (Felis catus), TNFSF15 is primarily produced by endothelial cells, macrophages, dendritic cells, and other immune cells in response to inflammatory stimuli or infection. TNFSF15 functions mainly by binding to its receptor death receptor 3 (DR3, TNFRSF25) expressed on T lymphocytes and other immune cells, activating intracellular pathways such as NF-κB and MAPK that regulate T cell activation, cytokine production, and inflammatory responses. In feline immune physiology, TNFSF15 is thought to contribute to regulation of immune-mediated inflammation and vascular responses, while also influencing T cell–mediated immunity. Similar to other mammals, TNFSF15 may play roles in chronic inflammatory diseases, immune regulation, and host responses to infection. Because TNF superfamily cytokines are important regulators of inflammation and immune signaling, feline TNFSF15 is of interest in veterinary immunology and inflammatory disease research, contributing to the understanding of cytokine-mediated immune regulation in cats.

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