Egyptian Rousette Bat CCL4 (MIP-1 beta) Recombinant Protein

Catalog Number:
RP2619BT
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Rousettus aegyptiacus (Egyptian rousette)
  • Bat CCL4 (C-C Motif Chemokine Ligand 4) (catalog RP2619BT) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it contains no affinity tags, is naturally endotoxin-free, and should be reconstituted in sterile PBS with at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids in length (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Egyptian rousette bat. It is stable for up to twelve months at -20 °C from receipt, with working aliquots (with carrier protein) stable for ~3 months; avoid repeated freeze/thaw cycles. The product is manufactured in the USA. It is commonly used to study chemokine-mediated immune responses, particularly macrophage and lymphocyte recruitment, inflammation, and cytokine signaling pathways; typical applications include chemotaxis and cell migration assays, immune cell activation studies, cytokine signaling research, ELISA and neutralization assays, flow cytometry and Western blot controls, and antibody development or validation. Kingfisher Biotech products are supplied for research applications only and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPMGSDPPTA CCFSYALRKI PRNFVVDYYE TSSLCSQPAV VFQTKRGRQV CANPSESWVQ EYMDDLELN (69)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Egyptian Rousette Bat (Rousettus aegyptiacus) CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine involved in recruitment and activation of immune cells during innate and antiviral immune responses. CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In R. aegyptiacus, CCL4 is likely produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes following stimulation by pathogen-associated molecules or inflammatory cytokines. This bat species is a known reservoir for viruses such as Marburg virus, making immune mediators like CCL4 important for understanding how immune cell trafficking is regulated during viral infection. Controlled expression of CCL4 may help recruit antiviral immune cells while limiting excessive inflammatory responses, contributing to the ability of Egyptian Rousette bats to tolerate viral infections with minimal pathology. Characterization of Egyptian Rousette Bat CCL4 supports studies of bat antiviral immunity, chemokine signaling, and host–pathogen interactions, providing insights into mechanisms of viral persistence and immune tolerance in natural reservoir hosts.

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