Slide 1 of 1 
Swine Annexin V Fluorescein
- Catalog Number:
- RPF0966S
- Availability:
- In stock
- Application:
- Flow Cytometry
- 100% Homology:
- Sus scrofa (pig)
- Annexins are important in various cellular and physiological processes such as providing a membrane scaffold, which is relevant to changes in the physical shape of the cell. Annexin proteins have been shown to be involved in trafficking and organization of vesicles, exocytosis, endocytosis and also calcium ion channel formation. Annexin proteins are not exclusively intracellular protein: Annexin proteins are also found in the extracellular space and have been linked to several processes fibrinolysis, coagulation, inflammation and apoptosis. Annexin proteins are all capable of binding negatively charged phospholipids in a calcium dependent manner and contain a 70 amino acid repeat sequence called an annexin repeat. Swine Annexin V Fluorescein has a predicted molecular weight of 36.0 kDa. Protien Sequence: MAQVLRGTVT DFPGFDERAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEIAVAFKTL FGRDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTDEKIL TEIIASRTPE ELRAIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDGRIDEA QVEQDAQALF QAGELKWGTD EEKFITILGT RSVSHLRRVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVMVSRSEID LFNIRKEFRK NFATSLYSMI KGDTSGDYKK ALLLLCGGED D (321).
| Amino Acid Sequence | MAQVLRGTVT DFPGFDERAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEIAVAFKTL FGRDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTDEKIL TEIIASRTPE ELRAIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDGRIDEA QVEQDAQALF QAGELKWGTD EEKFITILGT RSVSHLRRVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVMVSRSEID LFNIRKEFRK NFATSLYSMI KGDTSGDYKK ALLLLCGGED D (321) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | 2°C to 8°C |
| Country Of Origin | USA |
- Swine Annexin V (also known as ANXA5, Annexin A5, placental anticoagulant protein I (PAP-I), lipocortin V, or anchorin CII) is a calcium-dependent phospholipid-binding protein belonging to the annexin family, which plays important roles in membrane organization, cellular signaling, inflammation, and regulation of apoptosis. In pigs (Sus scrofa domesticus), Annexin V binds with high affinity to phosphatidylserine exposed on the outer leaflet of the plasma membrane during early stages of apoptosis or cellular injury in the presence of calcium ions. Because of this property, FITC-labeled swine Annexin V (Annexin V-FITC) is widely used in flow cytometry and fluorescence microscopy assays to detect and quantify apoptotic cells. Conjugation with fluorescein isothiocyanate (FITC) enables fluorescent labeling of cells displaying externalized phosphatidylserine, allowing sensitive identification of early apoptotic cells, typically used alongside viability dyes such as propidium iodide (PI) or 7-AAD to distinguish early apoptosis from late apoptosis or necrosis. In swine health and research, Annexin V-FITC assays are used to study immune cell apoptosis during viral infections such as porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza, as well as to evaluate inflammatory responses, tissue injury, and drug-induced cytotoxicity. It is also applied in reproductive biology to assess sperm membrane integrity and early embryonic cell viability in assisted reproductive technologies. As a biomarker of apoptosis and membrane remodeling, swine Annexin V supports veterinary and translational research into host-pathogen interactions, immune regulation, and cell death pathways relevant to both livestock health and human disease models.
10 ug (100 tests)
Price: $200.00
CITATIONS
Schmaltz L, Barakat E, Fleurot R, Uzbekov R, Reynaud K, Laffont L, Tsikis G, Mérour I, Mermillod P, Saint-Dizier M. Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs. Eur J Cell Biol. 2024 Dec 17;104(1):151471. doi: 10.1016/j.ejcb.2024.151471. Epub ahead of print. PMID: 39700614.
Application: Confocal Microscopy, Flow Cytometry
Sample Type: Cells (Spermatozoa)